Welcome to riboSeed’s documentation!¶
Impatient? See our Quickstart Guide
A brief overview of the theory can be found here.
The manuscript can be found here: Nicholas R Waters, Florence Abram, Fiona Brennan, Ashleigh Holmes, Leighton Pritchard; riboSeed: leveraging prokaryotic genomic architecture to assemble across ribosomal regions, Nucleic Acids Research, Volume 46, Issue 11, 20 June 2018, Pages e68, https://doi.org/10.1093/nar/gky212
riboSeed is an supplemental assembly refinement method to try to address the issue of multiple ribosomal regions in a genome, as these create repeates unresolvable by short read sequencing. It takes advantage of the fact that while each region is identical, the regions flanking are unique, and therefore can potentially be used to seed an assembly in such a way that rDNA regions are bridged.
- Choosing an Appropriate Reference
- riboSeed Pipeline
- Accessory Scripts